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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has long been observed in several tumors, which ends up in chromosome instability, and thus might lead to tumorigenesis. This protein was uncovered to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in cell-cycle controlled histone gene expression and performs a important position in marketing cell-cycle development from the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are explained. Two extra splice variants were being documented but comprehensive nucleotide sequence information and facts is just not however obtainable.
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Blocking peptides are peptides that bind especially for the goal antibody and block antibody binding. These peptide commonly contains the epitope recognized with the antibody.
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This tutorial outlines additional achievable optimization of cellular and immunoassay parameters to make certain the best possible benefits are...
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This guideline outlines further feasible optimization of cellular and immunoassay parameters to make sure the best possible outcomes are...
Antibodies bound to the blocking peptide now not bind into the epitope over the focus on protein. By comparing the staining in the blocked antibody vs . the antibody alone, you can see which staining is particular.
Target Information and facts Cyclin E belongs to your very conserved cyclin family members, whose associates are characterised by a remarkable periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Unique cyclins exhibit unique expression and degradation patterns which lead to the temporal coordination of each and every mitotic event. Cyclin E kinds a posh with and functions for a regulatory subunit of CDK2, whose activity is needed for mobile cycle G1/S changeover. This protein accumulates in the G1-S period boundary and is particularly degraded as cells progress by way of S period.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both equally antibodies...
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